U-Blot® Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb

U-Blot® Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb
U-Blot® Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb
$288.00
Size:100μl
SKU: WT9081-100

Catalog No.: WT9081

Specification: 100μL/300μL

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers. Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity. Phosphorylation by cdc2 controls the timing of cytokinesis.

Product Overview

Catalog No.
WT9081
SKU
WT9081-100
Category
Phosphorylation
Product Type
Other
Size
100μL/300μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
Store at -20°C. Avoid repeated freeze-thaw cycles
Research Use Only
Yes

Additional Specifications

Antibody Type
Recombinant monoclonal
Target
Phospho-Myosin Light Chain 2 (Ser19)
Reactivity
Human
Application
WB, ICC/IF
MW(Calculated)
20 kDa
MW(Observed)
18 kDa
Host Species
Mouse
Isotype
IgG1/Kappa
Conjugate
Unconjugated
Modification
Phosphorylation
Modification Site
Ser19
Recommended dilution ratio
WB 1:1000；ICC/IF 1:50
Composition
PBS, Glycerol, BSA
Purification process
Protein A affinity purification
storageCondition
Store at -20°C. Avoid repeated freeze-thaw cycles
Stability
Stable for 12 months from date of receipt / reconstitution.
Concentration
--
Clonal
Recombinant monoclonal
Clone Number
W-R54
Immunogen
polypeptide
Gene Name
MYL2
Protein Name
Myosin Light Chain 2 / Myosin regulatory light chain
Alternative name
--
Organism-1
Human
Gene ID
4633
SwissProt-1
P24844
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Background：
Background
Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers. Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity. Phosphorylation by cdc2 controls the timing of cytokinesis.
Cellular Localization
Cytoplasm, cytoskeleton
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Background：Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner. This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction. ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers. Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity. Phosphorylation by cdc2 controls the timing of cytokinesis.
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Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
Store at -20°C. Avoid repeated freeze-thaw cycles
06

Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

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Keywords:Phosphorylation