- Size
- 8 Sample Kit
- Species
- Human
- Compatible Sample Types
- Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
- Quantitative/Semi-Quantitative
- Semi-Quantitative
- Number of Targets Detected
- 12
- Solid Support
- Membrane
- Design Principle
- Sandwich-based
- Method Of Detection
- Chemiluminescence
- Research Area
- Post-Translational Modifications, Phosphorylation, HER/ErbB Signaling, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, Notch Signaling
- Introduction
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- Product Features
- Easy to use;No specialized equipment needed;Compatible with nearly any liquid sample;Proven technology (many publications);Highly sensitive (pg/ml);Sandwich ELISA specificity;Higher density than ELISA, Western blot or bead-based multiplex
- Suggested Application
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- Kit Components
- Human JAK/STAT Pathway Phosporylation Array C1 Membranes;Blocking Buffer;Detection Antibody Cocktail 1,000X HRP-Anti-Rabbit IgG Concentrate ;20X Wash Buffer I Concentrate ;20X Wash Buffer II Concentrate; 2X Cell Lysis Buffer Concentrate;Detection Buffer C;Detection Buffer D;8-Well Incubation Tray w/ Lid;Protease Inhibitor Cocktail;100x Phosphatase Inhibitor Cocktail I;Phosphatase Inhibitor Cocktail II;Plastic Sheets;Array Map Template;Manual
- Other Materials Required
- Pipettors, pipet tips and other common lab consumables;Orbital shaker or oscillating rocker;Tissue Paper, blotting paper or chromatography paper;Adhesive tape or Saran Wrap;Distilled or de-ionized water;A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system
- Protocol Outline
- Block membranes ;Incubate with Sample ;Incubate with Detection Antibody Cocktail; Incubate with HRP-Conjugated anti-IgG Incubate with Detection Buffers; Image with chemiluminescent imaging system ;Perform densitometry and analysis
- Storage/Stability
- For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.