U-Blot® Human Protein Oxidation Array G1

Protein Oxidation has been linked to aging, cancer and other diseases. The Human Protein Oxidation Antibody Array 1 is specifically designed for simultaneous identification of the relative levels of oxidation of 507 different human proteins in cell lysate, culture supernatant, serum, plasma and other biological samples. By monitoring the changes in protein oxidation in your experimental model system, you can verify oxidative damage without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot. The Human Protein Oxidation Antibody Arrays utilizes the 'biotin probes' method to biotin label carbonyl groups of oxidized proteins. In this method, the biotin probes first react with carbonyl groups of oxidized proteins to form the unstable Schiff bases, which are then further reduced to more stable amines. The biotin labeled sample then is added into antibody array glass slide wells. The antibody array slide wells are washed. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.