- Size
- 4 Sample Kit
- Species
- Mouse
- Compatible Sample Types
- Tissue Lysates, Cell Lysates
- Quantitative/Semi-Quantitative
- Semi-Quantitative
- Number of Targets Detected
- 55
- Solid Support
- Membrane
- Design Principle
- Sandwich-based
- Method Of Detection
- Chemiluminescence
- Research Area
- Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, TGF-beta Signaling, NFkB Signaling, Akt Signaling
- Introduction
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- Product Features
- Easy to use;No specialized equipment needed;Compatible with nearly any liquid sample;Proven technology (many publications);Highly sensitive (pg/ml);Sandwich ELISA specificity;Higher density than ELISA, Western blot or bead‑based multiplex
- Suggested Application
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- Kit Components
- Antibody Arrays;Blocking Buffer;Detection Antibody Cocktail;HRP‑Anti‑Rabbit IgG Concentrate;Wash Buffer I Concentrate;Wash Buffer II Concentrate;Cell Lysis Buffer Concentrate;Detection Buffer C;Detection Buffer D;8‑Well Incubation Tray w/ Lid;Protease Inhibitor Cocktail;Phosphatase Inhibitor Cocktail I;Phosphatase Inhibitor Cocktail II;Plastic Sheets;Array Map Template
- Other Materials Required
- Pipettors, pipet tips and other common lab consumables;Orbital shaker or oscillating rocker;Tissue Paper, blotting paper or chromatography paper;Adhesive tape or plastic wrap;Distilled or de‑ionized water;A chemiluminescent blot documentation system
- Protocol Outline
- Block membranes;Incubate with Sample;Incubate with Detection Antibody Cocktail;Incubate with HRP‑Conjugated anti‑IgG;Incubate with Detection Buffers;Image with chemiluminescent imaging system;Perform densitometry and analysis
- Storage/Stability
- Store kit at ≤ -20°C immediately upon arrival. Kit must be used before the 6 month expiration date.