U-Blot® Lectin Array 95

U-Blot® Lectin Array 95
U-Blot® Lectin Array 95
$9,999.00
SKU: WT9612A-95-12

Specification: 12 Sample Kit

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

Product information

Lectins are glycan-binding proteins that have been purified from trees, beans, and some fruits. They are highly specific for a given glycan based on their sequence and the different sugar unit structures the glycan contains. The Lectin Array 95 consists of a standard glass slide spotted with 12 identical lectin arrays (1 per well), each containing 95 unique lectins spotted in duplicate, plus positive controls. The slide comes with a 16-well removable gasket which allows for the processing of 12 samples using one slide. Four slides can be nested into a tray, which matches a standard microplate and allows for the automated robotic high-throughput processing of 48 arrays simultaneously. The Lectin Array 95 array provides a powerful new tool for glycosylation determination, drug discovery, and biomarker development, all while requiring limited sample volumes.

Product Overview

Catalog No.
WT9612A-95-12
SKU
WT9612A-95-12
Category
Glycosylation
Product Type
Other
Size
12 Sample Kit
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Research Use Only
Yes

Additional Specifications

Size
12 Sample Kit
Species
--
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative
Semi-Quantitative
Number of Targets Detected
95
Solid Support
Glass Slide
Design Principle
Sandwich-based
Method Of Detection
Fluorescence Laser Scanner
Research Area
Post-Translational Modifications, Glycosylation
Introduction
Lectins are glycan-binding proteins that have been purified from trees, beans, and some fruits. They are highly specific for a given glycan based on their sequence and the different sugar unit structures the glycan contains. The Lectin Array 95 consists of a standard glass slide spotted with 12 identical lectin arrays (1 per well), each containing 95 unique lectins spotted in duplicate, plus positive controls. The slide comes with a 16-well removable gasket which allows for the processing of 12 samples using one slide. Four slides can be nested into a tray, which matches a standard microplate and allows for the automated robotic high-throughput processing of 48 arrays simultaneously. The Lectin Array 95 array provides a powerful new tool for glycosylation determination, drug discovery, and biomarker development, all while requiring limited sample volumes.
Product Features
High sensitivity and specificity;Low sample volume (10-100 µl per array);Large dynamic range of detection;Compatible with most sample types;Test 4 or 8 samples on each slide;Suitable for high-throughput assays
Suggested Application
Identify and profile the glycans in their samples;Determine whether their biomarker of interest has glycan moieties;Find specific glycan binding ligands in biological samples
Kit Components
Dialysis Vials;Labeling Reagent;Labeling Buffer;Stop Solution;Lectin Array Glass Slide Assembly;Sample Diluent;20X Wash Buffer I;20X Wash Buffer II;Cy3 equivalent dye‑conjugated Streptavidin;Slide Washer/Dryer;Adhesive device sealer;Floating Dialysis Rack;Manual
Other Materials Required
Detection antibodies of interest (For sandwich‑based method only);Orbital shaker;Laser scanner for fluorescence detection;Aluminum foil;1.5ml Polypropylene microcentrifuge tubes;KCl, NaCl, KH₂PO₄ and Na₂HPO₄ (For label‑based method only);Plastic or glass containers, beaker, stir plate and stir bar;Pipettors, pipette tips, ddH₂O and other common lab consumables
Protocol Outline
Dry the glass slide;Block array surfaces;Incubate samples (samples need to be biotinylated for the label‑based approach);For the sandwich‑based principle; incubate with a detection antibody cocktail;For the label‑based principle; incubate the labeled‑streptavidin;Incubate with Cy3 Equivalent Dye‑Streptavidin;Disassemble the glass slide;Scan with a gene microarray laser scanner;Perform densitometry and analysis
Storage/Stability
Upon receipt, all components of the Lectin Array 95 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.

Tech Support

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Keywords:Glycosylation