- Size
- 48 Sample Kit
- Species
- --
- Compatible Sample Types
- Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
- Quantitative/Semi-Quantitative
- Semi-Quantitative
- Number of Targets Detected
- 95
- Solid Support
- Glass Slide
- Design Principle
- Sandwich-based
- Method Of Detection
- Fluorescence Laser Scanner
- Research Area
- Post-Translational Modifications, Glycosylation
- Introduction
- Lectins are glycan-binding proteins that have been purified from trees, beans, and some fruits. They are highly specific for a given glycan based on their sequence and the different sugar unit structures the glycan contains. The Lectin Array 95 consists of a standard glass slide spotted with 12 identical lectin arrays (1 per well), each containing 95 unique lectins spotted in duplicate, plus positive controls. The slide comes with a 16-well removable gasket which allows for the processing of 12 samples using one slide. Four slides can be nested into a tray, which matches a standard microplate and allows for the automated robotic high-throughput processing of 48 arrays simultaneously. The Lectin Array 95 array provides a powerful new tool for glycosylation determination, drug discovery, and biomarker development, all while requiring limited sample volumes.
- Product Features
- High sensitivity and specificity;Low sample volume (10-100 µl per array);Large dynamic range of detection;Compatible with most sample types;Test 4 or 8 samples on each slide;Suitable for high-throughput assays
- Suggested Application
- Identify and profile the glycans in their samples;Determine whether their biomarker of interest has glycan moieties;Find specific glycan binding ligands in biological samples
- Kit Components
- Dialysis Vials;Labeling Reagent;Labeling Buffer;Stop Solution;Lectin Array Glass Slide Assembly;Sample Diluent;20X Wash Buffer I;20X Wash Buffer II;Cy3 equivalent dye‑conjugated Streptavidin;Slide Washer/Dryer;Adhesive device sealer;Floating Dialysis Rack;Manual
- Other Materials Required
- Detection antibodies of interest (For sandwich‑based method only);Orbital shaker;Laser scanner for fluorescence detection;Aluminum foil;1.5ml Polypropylene microcentrifuge tubes;KCl, NaCl, KH₂PO₄ and Na₂HPO₄ (For label‑based method only);Plastic or glass containers, beaker, stir plate and stir bar;Pipettors, pipette tips, ddH₂O and other common lab consumables
- Protocol Outline
- Dry the glass slide;Block array surfaces;Incubate samples (samples need to be biotinylated for the label‑based approach);For the sandwich‑based principle; incubate with a detection antibody cocktail;For the label‑based principle; incubate the labeled‑streptavidin;Incubate with Cy3 Equivalent Dye‑Streptavidin;Disassemble the glass slide;Scan with a gene microarray laser scanner;Perform densitometry and analysis
- Storage/Stability
- Upon receipt, all components of the Lectin Array 95 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.