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Protein methylation refers to the covalent modification of lysine and arginine residues by the addition of methyl groups derived from S-adenosylmethionine (SAM). Lysine methylation, including mono-, di-, and trimethylation (Kme1, Kme2, and Kme3), serves as a key epigenetic mechanism controlling chromatin organization and gene expression. Analysis of protein methylation provides insights into epigenetic regulation and the molecular mechanisms underlying cellular identify and function.