UcallM® TCR/BCR-seq Kit

UcallM® TCR/BCR-seq Kit is designed for adaptive-immune-repertoire profiling. The T-cell-receptor (TCR) and B-cell-receptor (BCR) repertoires collectively mirror immune-system status under physiological or pathological challenges. This kit enriches and amplifies CDR-region repertoires of T / B lymphocytes for high-throughput sequencing, enabling deep profiling of immune diversity, clonal evolution and disease-associated signatures.

Applications 1.Tumor-immunology & tumor-microenvironment research: trace clonal dynamics of tumor-infiltrating lymphocytes 2.Autoimmune-disease and infectious-disease research 3.Immunotherapy monitoring: evaluate clonal expansion and resistance mechanisms post-CAR-T / TCR-T treatment

Measured Data

Figure 1-49. Amino Acid Motif Frequency Distribution in CDR3 Region

Figure 1-50. Overlapping Circos Plot

Figure 1-51. Circular Plot of VJ Gene Usage

Figure 1-52. Group Comparison of VJ Gene Usage

Figure 1-53. 3D Bar Chart for Immune Repertoire Diversity Analysis

Figure 1-54. Three-layer Ring Plot of Immune Repertoire Clonotypes

Figure 1-55. Rarefaction Curve of Sample Diversity versus Sample Size

Figure 1-56. Scatter Plot of Shared Clonotypes Application Case Cell Rep Med: Variant-Specific mRNA Vaccines Elicit Robust Immune Responses and Effectively Protect Mice against SARS-CoV-2 and Its Variants (DOI: 10.1016/j.xcrm.2022.100634) This study investigated the immune response and protective efficacy of lipid nanoparticle-mRNA (LNP-mRNA) vaccines specifically developed against SARS-CoV-2 variants (B.1.351/Beta and B.1.617/Delta).

Figure 1-57. Immune repertoire sequencing was applied in this study to analyze TCR/BCR diversity and clonal expansion.