UcallM® TCR-seq Kit

UcallM® TCR-seq Kit is a library construction kit dedicated to T cell receptor (TCR) immune repertoire sequencing. Combined with high-throughput sequencing and data analysis, it can accurately capture the full-length sequence of TCR complementarity-determining region 3 (CDR3) (covering α/β/γ/δ chains), comprehensively resolve T cell clonal diversity, rearrangement patterns, clonal evolution dynamics and other information, helping to unlock the in-depth mechanism of T cell-mediated immune responses.

Application Scenarios 1.Tumor immunity / microenvironment research: track clonal evolution and antigen-specific evolution of tumor-infiltrating T cells, evaluate immune therapeutic response 2.Autoimmune disease and infectious immunity research: dissect T cell immune response mechanisms under disease states 3.T cell therapy development: provide tools for clonal dynamic monitoring and target validation for TCR-T / CAR-T therapy

Application Case Ann Rheum Dis: HMGCR-Reactive CD4+ T Cells Participate in Anti-HMGCR Immune-Mediated Necrotizing Myopathy (DOI:10.1136/ard-2024-225732) This study revealed that HMGCR-specific CD4+ T cells exist in patients with anti-HMGCR antibody-positive immune-mediated necrotizing myopathy (IMNM). These cells can be detected in both the patient’s circulatory system and affected muscle tissue, suggesting that these cells may participate in disease initiation and progression.

Figure 1-58. In this study, TCR-seq was used to track TCRs and verify that pathogenic HMGCR-reactive CD4+ T cells infiltrate muscle tissue. It also identified shared clonotypes across patients, providing direct evidence for disease mechanisms.