UcallM® RiboRNA Depletion Kit (Human/Mouse/Rat)

UcallM® RiboRNA Depletion Kit (Human/Rat/Mouse) is built on new-generation high-efficiency rRNA targeted depletion reagents. Innovative technology breaks key bottlenecks of RNA-seq library preparation. This kit adopts targeted reverse transcription inhibition strategy, which precisely blocks rRNA and/or Globin mRNA during reverse transcription and cDNA synthesis. Only a 20-min single-step operation removes over 80% of cytoplasmic and mitochondrial rRNA from total RNA, while fully preserving the integrity of mRNA, non-coding RNA, circular RNA and other target molecules. Rigorous validation demonstrates >90% rRNA depletion efficiency in human samples with excellent batch stability (CV＜5%). Compatible with Illumina, Thermo Fisher and other mainstream library construction kits. Compared with traditional probe hybridization or RNase H methods, it significantly increases valid read proportion and lowers sequencing cost, providing an efficient and reliable solution for transcriptome research and high-throughput experiments.

Experimental Workflow

Product Highlights 1.Simplified workflow: directly integrated into standard first-strand cDNA synthesis system, no multi-step purification required 2.Broad compatibility: applicable for 1 ng~1 μg/μL intact or degraded RNA samples, including FFPE and other clinically processed samples 3.High specificity: retention rate of non-target RNA >95%, information loss minimized

Figure 1-68. Sequencing data comparison of libraries prepared with rRNA depletion Notes 1.DNase I treatment and purification are recommended for total RNA samples 2.RNase-free consumables are recommended; use nuclease-free water 3.rRNA Depletion Probes can efficiently and stably deplete rRNA. Libraries prepared following standard workflow. If library yield is low (caused by more thorough rRNA removal), an extra round of amplification can be added. If adapter dimers appear, perform secondary magnetic bead purification to optimize results. Customer Publications 1.Advanced Science: Targeting m⁶A Reader IGF2BP2 Suppresses Acute Myeloid Leukemia by Regulating Glutamine Metabolism (DOI:10.1002/advs.202406332) This study investigated the role of m⁶A methyltransferase METTL16 in male meiosis, focusing on its regulation of sex chromosome inactivation (MSCI), double-strand break (DSB) formation, homologous recombination and expression of key meiotic genes.

Figure 1-69. This study used Ribo-seq to generate ribosome profiling maps for efficient enrichment of ribosome-protected mRNA or small RNA. UcallM® RiboRNA Depletion Kit was used to specifically deplete rRNA with high efficiency and specificity to reduce interference from off-target RNA.

2.Nature Communications: YTHDF2 Enhances Anti-Tumor Immune Function of CD8 T Cells by Coordinating Epithelial Transcription and Transcriptional Networks (DOI:10.1038/s41467-024-53997-6) This study revealed that RNA m⁶A reader YTHDF2 enhances multi-functional anti-tumor immunity of CD8 T cells via dual mechanisms of coordinating epitranscription (RNA modification) and transcription (chromatin regulation).

Figure 1-70. Epibiotech rRNA depletion kit was applied in Ribo-seq to specifically remove rRNA so as to enrich RPFs. This step ensures sequencing data focus on actively translated mRNA regions and avoids rRNA interference, providing high signal-to-noise data for translational analysis.