U-Blot® EGFR (Phospho Tyr845) Rabbit mAb

U-Blot® EGFR (Phospho Tyr845) Rabbit mAb
U-Blot® EGFR (Phospho Tyr845) Rabbit mAb
$288.00
Size:50μL
SKU: WR5101-50

Catalog No.: WR5101

Specification: 50μL/100μL

Stock: In stock

U-Blot® EGFR (Phospho Tyr845) Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],

Product Overview

Catalog No.
WR5101
SKU
WR5101-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
EGFR
Reactivity
Human
Application
WB,IF,ELISA
MW(Calculated)
134kD
MW(Observed)
200kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Phospho
Modification site
Tyr845
Recommended Dilution Ratio
WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
PT1384R
Immunogen
--
Sequence
--
Specificity
EGFR (Phospho Tyr845) Antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr845.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):VLvKT
Gene Name
EGFR ERBB ERBB1 HER1
Protein Name
Epidermal growth factor receptor
Other Name
EGFR;ERBB;ERBB1;HER1;Epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1
Organism-1
Human
Gene ID-1
1956
SwissProt-1
P00533
Organism-2
Mouse
Gene ID-2
13649
SwissProt-2
Q01279
Organism-3
--
Gene ID-3
--
SwissProt-3
--
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Disease:Defects in EGFR are associated with lung cancer [MIM:211980].,Function:Isoform 2/truncated isoform may act as an antagonist.,Function:Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin.,miscellaneous:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.,online information:EGFR entry,PTM:Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.,PTM:Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1.,tissue specificity:Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.,

Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

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Keywords:Rabbit mAbs