U-Blot® Acetyl Lysine Rabbit mAb

Experimental Conditions:

Cell line: HeLa

Treatment: 1 μM MS-275 for 24 h

Fixation:4% Paraformaldehyde, 15 min at room temperature

Permeabilization: 0.1% Triton X-100, 15 min at room temperature

Blocking: 5% BSA, 30 min at room temperature

Primary antibody: Pan-Acetyllysine Rabbit mAb (WR5298, 1:200)

Primary antibody incubation: 1 h at room temperature

Secondary antibody: U-Blot® Goat Anti Rabbit IgG (H&L)-AbFluor 647 (WS0919), 1 h at room temperature

Conterstaining: Ucell® Actin Green 488 Phalloidin/NucleiBlue Dual Staining Kit (WFP035)

Results:

Representative immunofluorescence images of HeLa cells treated with 1 μM MS-275 for 24 h and stained with Pan-Acetyllysine Rabbit mAb (magenta), phalloidin (green), and DAPI (blue). Pan-acetyllysine immunoreactivity was predominantly detected in the nucleus. Phalloidin and DAPI were used to visualize F-actin and nuclei, respectively.