U-Blot® Histone H2A.X (Acetyl Lys9) Rabbit mAb

Background: Histone H2A.X is an essential H2A variant primarily responsible for genome stability and cellular DNA damage response. Upon DNA double-strand breaks, H2A.X undergoes C-terminal phosphorylation to form γH2A.X, which initiates the recruitment of multiple repair factors and activates DNA damage checkpoints. Dynamic lysine acetylation on the N-terminal tail of H2A.X cooperates with phosphorylation to regulate chromatin remodeling, DNA repair efficiency and basal transcription. H2A.X Lys9 acetylation (H2A.X K9ac) is modified by p300/CBP and TIP60 complexes. Under normal physiological conditions, this mark accumulates on promoters of DNA repair and proliferation-related genes to maintain open chromatin status. After DNA damage, H2A.X K9ac enriches at damage foci to assist chromatin remodeling and repair factor recruitment. Impaired H2A.X K9 acetylation delays the clearance of DNA damage signaling.