U-Blot® Histone H3 (Di Methyl Lys14) Rabbit mAb

U-Blot® Histone H3 (Di Methyl Lys14) Rabbit mAb
U-Blot® Histone H3 (Di Methyl Lys14) Rabbit mAb
$700.00
Size:100μL
SKU: WT8420-100

Catalog No.: WT8420

Specification: 100μL/300μL

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background: Histone H3 forms H3-H4 tetramers as nucleosome core scaffold. Lysine dimethylation is a reversible epigenetic modification controlled by methyltransferases and demethylases. Different dimethyl marks recruit specific reader proteins to regulate poised transcription, heterochromatin formation, DNA repair and cell differentiation. Each lysine site on H3 carries unique biological functions. H3K14me2 enriches at immune and metabolic gene promoters. It balances acetylation-mediated transcription activation and maintains stable basal gene expression. Upon oxidative damage, H3K14me2 accumulates at lesion sites to coordinate chromatin repair.

Product Overview

Catalog No.
WT8420
SKU
WT8420-100
Category
Histone Modification Antibodies
Product Type
Other
Size
100μL/300μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-20.0°C
Research Use Only
Yes

Additional Specifications

Target
Dimethylated Histone H3 Lysine 14, H3K14me2
Reactivity
Human Vertebrates
Application
WB, ELISA, Luminex
MW(Calculated)
15kD
MW(Observed)
--
Host Species
Rabbit
Isotype
Rabbit IgG
Conjugate
Unconjugated
Modification
Di Methyl
Modification site
Lys14
Recommended dilution ratio
WB 0.25 ug/mL – 1 ug/mL; ELISA 0.2 ug/mL - 1 ug/mL; Luminex 0.1 ug/mL – 0.5 ug/mL.
Formulation
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Source
--
Purification
Protein A affinity purified from an animal origin-free culture supernatant
Purity
--
Storage
-20.0°C
Concentration
1 mg/mL
Clonality
Recombinant Monoclonal
Clone Number
WR-201
Immunogen
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys14).
Sequence
--
Specificity
This antibody reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1) or trimethylated Lysine 14 (K14me3), or other methylation in histone H3.
Gene name
--
Protein name
--
Alternative name
H3K14me2
Organism-1
Human
Gene ID‑1
8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
SwissProt-1
P68431;Q71DI3;P84243
Organism-2
Mouse
Gene ID‑2
319152;15077;15078
SwissProt-2
--
Organism-3
Rat
Gene ID‑3
291159;100361558
SwissProt-3
Q6LED0;P84245
Organism-4
--
Gene ID‑4
--
SwissProt-4
--
Cellular Localization
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Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-20.0°C
03

Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

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HoursMonday-Friday 09:00-18:00 CST

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Keywords:Histone Modification Antibodies