U-Blot® Histone H3 (Mono Methyl Lys27) Mouse mAb

U-Blot® Histone H3 (Mono Methyl Lys27) Mouse mAb
U-Blot® Histone H3 (Mono Methyl Lys27) Mouse mAb
$700.00
Size:100μL
SKU: WT8579-100

Catalog No.: WT8579

Specification: 100μL/300μL

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Product Overview

Catalog No.
WT8579
SKU
WT8579-100
Category
Histone Modification Antibodies
Product Type
Other
Size
100μL/300μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-20°C/2 year. Avoid freeze/thaw cycles.
Research Use Only
Yes

Additional Specifications

Target
Histone H3
Reactivity
Human, Wide Range Predicted
Application
CUT&Tag,ChIP,ChIP-Seq,WB,IF,DB,ICC
MW(Calculated)
--
MW(Observed)
17 kDa
Host Species
Mouse
Isotype
IgG2a
Conjugate
Unconjugated
Modification
Monomethyl
Modification site
Lys27
Recommended dilution ratio
ChIP 2 - 5 µg per ChIP; ChIP-Seq 2 - 5 µg each; WB 0.5 - 2 µg/ml dilution; CUT&Tag 1-2 µg per 50 µl reaction
Formulation
PBS pH 7.5 containing 30% glycerol. 0.3M NaCl, and 0.035% sodium azide.
Source
--
Purification
Protein G Chromatography
Purity
--
Storage
-20°C/2 year. Avoid freeze/thaw cycles.
Concentration
1.0 µg/µl
Clonality
Monoclonal
Clone Number
--
Immunogen
This Histone H3 monomethyl Lys27 antibody was raised against a peptide containing monomethyl Lys27 of human Histone H3.
Sequence
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Specificity
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Gene name
--
Protein name
--
Alternative name
H3K27me1
Organism-1
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Gene ID‑1
--
SwissProt-1
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Organism-2
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Gene ID‑2
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SwissProt-2
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Organism-3
--
Gene ID‑3
--
SwissProt-3
--
Organism-4
--
Gene ID‑4
--
SwissProt-4
--
Cellular Localization
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Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-20°C/2 year. Avoid freeze/thaw cycles.
03

Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

WeChat / IMUcallm-Tech

HoursMonday-Friday 09:00-18:00 CST

Request Technical Support
Keywords:Histone Modification Antibodies