Itaconate‑Mediated Modifications

Introduction

Itaconate is an immunometabolite that drives two distinct protein modifications: cysteine itaconation (S‑itaconation) and lysine itaconylation (K‑itaconylation). S‑itaconation was first characterized in 2018 as an electrophilic cysteine modification involved in inflammatory regulation. In 2023, K‑itaconylation was identified as a novel lysine acylation modification. Despite sharing the same metabolic origin, these modifications are mediated through distinct chemical mechanisms and contribute to different biological processes.

Feature	S‑Itaconation	K‑Itaconylation
Modified Residue	Cysteine (Cys)	Lysine (Lys)
Precursor / Donor	Itaconate	Itaconyl‑CoA
Chemical Mechanism	Cysteine thiol modification	Lysine acylation
Reversibility	Generally stable	Not fully characterized
Biological Functions	Inflammatory regulation, antibacterial immunity	Not fully characterized

Products Soutions

Our antibodies enable detection of both cysteine itaconation (S‑itaconation) and lysine itaconylation (K‑itaconylation), providing tools for studying distinct itaconate‑mediated protein modifications.

• Cysteine itaconation detection
• Lysine itaconylation detection
• Specific enrichment of K‑itaconylated proteins

Product List

Product Type	Product Name	Catalog No.
Rabbit pAb	PanItaconylCysteine Rabbit pAb	WT9030
Rabbit mAb	PanItaconylCysteine Rabbit mAb	WT9031
MultiPTM	ItaconylCysteine MultiPTM Rabbit Monoclonal Antibody mix	WT9031M
Agarose Conjugated	Itaconyllysine Specific Antibody Conjugated Agarose Beads	WT9160B

Research Areas

• Inflammatory regulation

References

1. Mills EL, Ryan DG, Prag HA, et al. Itaconate is an anti‑inflammatory metabolite that activates Nrf2 via alkylation of KEAP1. Nature. 2018;556(7699):113‑117.
2. Liu D, Xiao W, Li H, et al. Discovery of Itaconate‑Mediated Lysine Acylation. J Am Chem Soc. 2023;145(23):12673‑12681.