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Tools for Immune Cell Research
| Research Stage | Common Tools |
| Cell Culture | Culture consumables, culture media, serum, cytokines |
| Cell Activation | CD3/CD28, LPS, cytokines |
| Cell Sorting | Magnetic beads, FACS |
| Phenotypic Characterization | Flow cytometry, antibodies |
| Gene Expression Analysis | ELISA, TR-FRET, qPCR, Western blot |
| Gene Regulation | siRNA, mRNA, CRISPR |
| Nucleic Acid Delivery | Transfection reagents, electroporation, viral vectors |
| Functional Assays | Cytotoxicity, Phagocytosis, Proliferation |
| Cell Imaging | Fluorescent probes, Immunofluorescence (IF), TSA |
| Cell Death Analysis | Apoptosis, Ferroptosis, Pyroptosis, etc. |
| 3D Research Models | Organoids, Spheroids, Co-culture |
References
[1]Medzhitov R. Recognition of microorganisms and activation of the immune response. Nature. 2007;449:819–826. DOI: 10.1038/nature06246.
[2]Ratajczak MZ, Kucia M. Hematopoiesis and innate immunity: an inseparable couple for good and bad times. Leukemia. 2022;36:23–32.
[3]Milsom MD, et al. Causes and Consequences of Hematopoietic Stem Cell Heterogeneity. Cell Stem Cell. 2018.
[4]Swann JW, Olson OC, Passegué E. Made to order: emergency myelopoiesis and demand-adapted innate immune cell production. Nature Reviews Immunology. 2024;24:596–613.
[5]Sonnenberg GF, Hepworth MR. Functional interactions between innate lymphoid cells and adaptive immunity. Nature Reviews Immunology. 2019;19:599–613.
[6]Schäfer PSL, et al. Integrating single-cell multi-omics and prior biological knowledge for a functional characterization of the immune system. Nature Immunology. 2024;25:405–417.
[7]Irac SE, et al. Single-cell immune repertoire analysis. Nature Methods. 2024;21:777–792.
Heras-Murillo I, et al. Dendritic cells as orchestrators of anticancer immunity and immunotherapy. Nature Reviews Clinical Oncology. 2024;21:257–277.
[8]Vivier E, et al. Natural killer cell therapies. Nature. 2024;626:727–736.
[9]Peng L, et al. CAR-T and CAR-NK as cellular cancer immunotherapy for solid tumors. Cellular & Molecular Immunology. 2024;21:1089–1108.
[10]Zitvogel L, Pitt JM, Daillère R, Smyth MJ, Kroemer G. Mouse models in oncoimmunology. Nature Reviews Cancer. 2016;16:759–773.
[11]Chuprin J, Buettner H, Seedhom MO, et al. Humanized mouse models for immuno-oncology research. Nature Reviews Clinical Oncology. 2023;20:192–206.
[12]Shultz LD, Brehm MA, Garcia-Martinez JV, Greiner DL. Humanized mice for immune system investigation: progress, promise and challenges. Nature Reviews Immunology. 2012;12:786–798.
[13]Guil-Luna S, Sedlik C, Piaggio E. Humanized Mouse Models to Evaluate Cancer Immunotherapeutics. Annual Review of Cancer Biology. 2021;5:119–136.
[14]Zhou Z, Pang Y, Ji J, et al. Harnessing 3D in vitro systems to model immune responses to solid tumours: a step towards improving and creating personalized immunotherapies. Nature Reviews Immunology. 2024;24:18–32.
[15]Polak R, Zhang ET, Kuo CJ. Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment. Nature Reviews Cancer. 2024;24:523–539. [16]Bar-Ephraim YE, Kretzschmar K, Clevers H. Organoids in immunological research. Nature Reviews Immunology. 2020;20:279–293.
[17]Drost J, Clevers H. Organoids in cancer research. Nature Reviews Cancer. 2018;18:407–418.
[18]Wang J, Tao X, Zhu J, et al. Tumor organoid-immune co-culture models: exploring a new perspective of tumor immunity. Cell Death Discovery. 2025;11:195.
[19]Dijkstra KK, et al. Generation of tumor-reactive T cells by co-culture of peripheral blood lymphocytes and tumor organoids. Cell. 2018 Sep 6;174(6):1586-1598.e12.
[20]Cattaneo CM, et al. Tumor organoid–T-cell coculture systems. Nature Protocols. 2020.Jan;15(1):15-39.
[21]Park JV, et al. Tumor Cells Modulate Macrophage Phenotype in a Novel In Vitro Co-Culture Model of the NSCLC Tumor Microenvironment. Journal of Thoracic Oncology. 2022.Oct;17(10):1178-1191.