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From RNA Modification Landscapes to Gene Expression Regulation — Empowering Comprehensive Investigation of RNA Metabolism, Post-Transcriptional Regulation & Cellular Adaptation
RNA molecules undergo extensive chemical modifications that dynamically regulate RNA stability, localization, translation efficiency, splicing, and degradation. Beyond serving as passive carriers of genetic information, modified RNAs represent a critical regulatory layer controlling gene expression programs across development, stress responses, immunity, metabolism, and disease progression.Epitranscriptomic regulation integrates RNA modification enzymes, RNA-binding proteins, RNA metabolic machinery, and signaling pathways to establish dynamic control of transcript fate.
Major RNA modification systems include:
· m6A RNA methylation and demethylation
· m5C RNA modification
· Pseudouridine (Ψ) modification
· m1A RNA modification
· m7G RNA modification
· RNA editing and transcriptome remodeling
· RNA stability and translation regulation
Dysregulation of RNA modification networks contributes to diverse biological and pathological processes, including:
· Cancer initiation and tumor evolution
· Immune cell differentiation and inflammatory responses
· Stem cell maintenance and cell fate determination
· Neurodevelopment and neurodegeneration
· Metabolic adaptation
· Aging and stress responses
Our validated antibody solutions enable researchers to decode RNA regulatory mechanisms by profiling:
· RNA modification abundance and distribution
· Writer, eraser, and reader enzyme networks
· RNA-binding protein regulation
· Translation and RNA stability pathways
· Epitranscriptomic remodeling during disease progression
RNA Modification & Epitranscriptomics Antibody Portfolio
—Comprehensive Solutions for RNA Modification Profiling, Regulatory Network Mapping & Functional Mechanism Discovery
RNA modification landscapes are established through coordinated activity of RNA modifying enzymes, RNA-binding proteins, and downstream regulatory pathways.
Our antibody portfolio provides validated tools targeting key molecular regulators involved in:
Research Focus | Representative Biomarkers | Research Applications |
m6A RNA Modification Machinery | METTL3, METTL14, WTAP, VIRMA/KIAA1429, RBM15, RBM15B,FTO, ALKBH5, YTHDF1, YTHDF2, YTHDF3, YTHDC1, IGF2BP1, IGF2BP2, IGF2BP3 | Profiling RNA methylation regulation, m6A deposition/removal, RNA stability, translation control, and disease-associated epitranscriptomic remodeling |
m5C RNA Modification Network | NSUN2, NSUN5, NSUN6, DNMT2, ALYREF, TET2 | Investigation of RNA cytosine methylation, RNA export, translation regulation, and cellular adaptation |
Pseudouridine Modification Pathway | DKC1, PUS1, PUS7, PUS10 | Analysis of Ψ RNA modification, ribosome function, RNA stability, and translational regulation |
m1A RNA Modification Network | TRMT6, TRMT61A, TRMT61B, ALKBH3, ALKBH1 | Investigation of m1A-mediated RNA regulation, mitochondrial RNA metabolism, and stress adaptation |
m7G RNA Modification System | METTL1, WDR4, RNMT, WBSCR22 | Characterization of RNA cap modification, tRNA stability, translation initiation, and ribosome biogenesis |
RNA Editing & Transcriptome Remodeling | ADAR1, ADAR2, APOBEC1 | Study of A-to-I RNA editing, transcriptome diversity, immune recognition, and neurological regulation |
RNA Stability & Decay Regulation | DCP1A, DCP2, XRN1, EXOSC10, UPF1, SMG6 | Analysis of RNA degradation pathways, nonsense-mediated decay, and transcript turnover |
Translation Regulation Network | eIF4E, eIF4G, eIF2α, p-eIF2α, RPS6, p-RPS6, mTOR, p-mTOR, AKT, p-AKT | Investigation of translational control, stress response, and protein synthesis regulation |
Epitranscriptomic Regulatory Network
—Master Regulatory Programs Controlling RNA Modification, Transcript Fate & Cellular Adaptation
RNA modification regulation is controlled by interconnected networks involving RNA modification enzymes, RNA-binding proteins, translation machinery, and stress-responsive signaling pathways.
Regulatory Network | Representative Targets | Biological Interpretation & Research Applications |
m6A Writer Complex Network | METTL3, METTL14, WTAP, VIRMA, RBM15, ZC3H13 , HAKAI/CBLL1 | Catalyzes m6A deposition on RNA transcripts; regulates RNA stability, translation efficiency, splicing, and cell fate decisions |
m6A Eraser Network | FTO, ALKBH5 | Controls reversible removal of m6A modification; regulates RNA metabolism, stem cell maintenance, cancer progression, and stress adaptation |
m6A Reader Network | YTHDF1, YTHDF2, YTHDF3, YTHDC1, IGF2BP1/2/3, YTHDC2 | Decodes m6A marks to regulate RNA translation, degradation, localization, and functional output |
RNA Processing & Splicing Network | HNRNPA2B1, HNRNPC, SRSF1, SRSF3 | Links RNA modifications with alternative splicing, RNA maturation, and transcript diversity |
Translation Control Network | eIF4E, eIF4G, eIF2α, p-eIF2α, mTOR, AKT | Connects RNA modification status with protein synthesis, nutrient sensing, and stress adaptation |
RNA Stability & Surveillance Network | UPF1, DCP2, XRN1, EXOSC10 | Controls RNA degradation, quality control, and transcriptome homeostasis |
Epigenetic–Epitranscriptomic Crosstalk Network | DNMT1, TET2, EZH2, HDAC1/2, SIRT1, DNMT3A , DNMT3B | Integrates DNA/histone regulation with RNA modification to coordinate gene expression programs |
Stress-Responsive RNA Remodeling Network | HIF-1α, NRF2, ATF4, p53 | Regulates adaptive RNA modification responses under hypoxia, oxidative stress, and metabolic stress |
Featured RNA Modification Research Panels
—Comprehensive Antibody Panels for Decoding RNA Modification Networks, Post-Transcriptional Regulation & Disease-Associated Molecular Remodeling
RNA regulation is governed by a multilayered network involving RNA modification enzymes, RNA-binding proteins, RNA processing machinery, translation regulators, and stress-responsive signaling pathways.
Integrated antibody profiling enables researchers to investigate how epitranscriptomic regulation controls RNA fate, protein expression, cellular adaptation, and disease progression.
n m6A RNA Modification Core Profiling Panel—Mapping RNA methylation dynamics and functional regulation
METTL3 | METTL14 | WTAP | FTO |
ALKBH5 | YTHDF1 | YTHDF2 | YTHDC1 |
n Stress-Responsive Epitranscriptomic Remodeling Panel—Investigating RNA Adaptation During Hypoxia, Oxidative Stress & Metabolic Challenges
METTL3 | FTO | ALKBH5 | YTHDF2 | ADAR1 |
HIF-1α | NRF2 | ATF4 | p53 |
|
n RNA Translation Regulation Panel—Connecting RNA modification with protein synthesis control
eIF4E | eIF4G | eIF2α | p-eIF2α | RPS6 |
mTOR | p-mTOR | AKT | p-AKT | p-RPS6 |
n RNA Stability & Degradation Panel—Decoding transcript turnover and RNA quality control
DCP1A | DCP2 | XRN1 | UPF1 | EXOSC10 | DIS3 |
n Disease-Associated Epitranscriptomic Remodeling Panel—Investigating RNA modification changes during disease progression
METTL3 | FTO | ALKBH5 | YTHDF2 |
ADAR1 | mTOR | p53 | NRF2 |
Why Researchers Choose Our RNA Modification & Epitranscriptomics Solutions
Validated RNA Regulatory Targets. Integrated Epitranscriptomic Networks. Mechanistic Insights into Gene Expression Control.
RNA regulation requires multidimensional characterization beyond RNA abundance measurement.
Our antibody portfolio enables researchers to investigate:
Research Challenge | Integrated Antibody Solution |
How are RNA modifications established? | RNA modification writer/eraser profiling — METTL3/METTL14/WTAP, FTO/ALKBH5, NSUN family, TRMT6/TRMT61A |
How are RNA modifications interpreted? | RNA modification reader profiling — YTHDF1/2/3, YTHDC1/2, IGF2BP1/2/3 |
How do RNA modifications affect translation? | Translation and signaling readouts — eIF4E, eIF2α/p-eIF2α, mTOR/p-mTOR, AKT/p-AKT, RPS6/p-RPS6 |
How are transcripts stabilized or degraded? | RNA surveillance profiling — DCP2, XRN1, UPF1, EXOSC10 |
How does epitranscriptomics interact with disease biology? | Integrated panels for cancer, immunity, metabolism, and neurobiology research |
How can spatial RNA regulation be studied? | Multiplex IF/IHC-compatible antibodies for spatial profiling of RNA modification regulators, RNA-binding proteins, and cellular signaling states |
Key reference
1. Wiener D, Schwartz S. The epitranscriptome beyond m6A. Nature Reviews Genetics. 2021;22:119–131.
2. Sun H, Li K, Liu C, et al. Regulation and functions of non-m6A mRNA modifications.Nature Reviews Molecular Cell Biology. 2023;24:714–731.
3. Deng X, Qing Y, Horne D, et al. The roles and implications of RNA m6A modification in cancer.Nature Reviews Clinical Oncology. 2023;20:507–526.Yang Y, Hsu PJ, Chen YS, Yang YG.Dynamic transcriptomic m6A decoration: writers, erasers, readers and functions.Trends Genet. 2018;34:783–799.
4. Murakami S, Jaffrey SR. Hidden codes in mRNA: Control of gene expression by m6A.Molecular Cell. 2022;82:2236–2251.
5. Lee AC. Epitranscriptomics of cancer microniches.Nature Reviews Cancer. 2023;23:189.