UcallM® One-Step mRNA Synthesis Kit

The UcallM® One-Step mRNA Synthesis Kit is built on innovative co-transcription technology, offering an all-in-one solution for efficient in-vitro synthesis of RNA-protein complexes or functional RNA molecules. With optimized reaction components and proprietary buffer mixtures, it accepts linear or circular DNA templates. Using NTPs as substrates, it transcribes DNA sequences downstream of the T7 promoter to generate mRNA. Incorporation of the naturally modified nucleotide N1-Me-pUTP reduces the immunogenicity of mRNA therapeutics and improves mRNA stability as well as protein expression capacity. It is suitable for multiple applications including CRISPR gRNA preparation, RNA aptamer screening, ribonucleoprotein (RNP) complex assembly and mRNA vaccine production.

Product Highlights 1.High-activity enzyme system High-purity and highly stable RNA polymerases (T7, SP6, T3, etc.) are adopted to greatly boost transcription efficiency, enabling large-scale RNA synthesis within a short incubation time (1–2 h). It supports synthesis of long RNA fragments (>1 kb) and minimizes truncated products caused by secondary structures. 2.High-purity RNA product Strict elimination of template DNA contamination (via DNase I treatment or template-free design); Residual dNTPs <0.1%, avoiding interference with downstream applications such as in-vitro translation and microinjection.

Performance Data High transcription yield: 1 μg template DNA yields 200 μg mRNA after 2 h incubation at 37 °C. As shown in agarose gel electrophoresis (Figure 1), mRNA transcribed with this kit exhibits brighter, broader and homogeneous bands with good reproducibility, no smearing and no mRNA degradation.

Figure 1-76. Electrophoresis results of UcallM® One-Step mRNA Synthesis Kit (Lane 1, 2); competitor reagent (Lane 3, 4). Table 1-1. High capping efficiency (LC-MS)