UcallM® FECTMAX mRNA Transfection Reagent

UcallM® FECTMAX mRNA Transfection Reagent is an innovative delivery reagent optimized specifically for mRNA. Using smart cationic lipid complex technology, it efficiently delivers mRNA into eukaryotic cells for both in vitro and in vivo applications. Positively charged lipid components tightly bind negatively charged mRNA to form stable complexes. Complexes enter cells via membrane fusion or endocytosis and release mRNA into the cytoplasm.

Core Advantages 1.Fast and robust expression: Protein expression can be detected as early as 6 h after transfection. 2.Sustained expression: mRNA-protecting technology extends expression duration up to 72 h in vitro. 3.Low cytotoxicity: Cell viability >95% at 48 h post-treatment; compatible with serum-free medium to prevent experimental artifacts. 4.No genomic integration risk: Transient expression only, suitable for preclinical research and gene therapy development. Notes 1.For optimal transfection efficiency, seed cells to reach 70–80% confluency on the day of transfection. 2.Form complexes in serum-free, antibiotic-free medium. Remove growth medium prior to complex addition. Recommended optimization ratio range: 1:1 ~ 1:8. Performance Data

Figure 1-80. Protein expression in 293T, A549 and HepG2 cells at 24 h after mRNA transfection.