UcallM® DNA Clean Beads

UcallM® DNA Clean Beads are high-performance magnetic beads developed based on the SPRI (Solid-Phase Reversible Immobilization) principle, designed for DNA fragment size selection and purification in NGS library construction. Manufactured with imported magnetic bead raw materials and finely optimized buffer systems, they are compatible with DNA/RNA library prep kits from all vendors, as well as restriction digestion, ligation and cloning reactions. They enable efficient size-selective purification of DNA fragments of defined lengths. The operating protocol is fully compatible with the widely used AMPure XP Beads. Fragment recovery efficiency and final library size distribution are highly comparable, making them an ideal choice for DNA processing in scientific research.

Storage: Store at 2–8 °C; 12-month shelf life. Do not freeze. Maintain low temperature with ice packs during shipment. Experimental Workflow

Product Highlights 1.Precise size-selection, flexible and tunable Dual-round size-selection: By adjusting bead-to-sample ratios (Table 1), target DNA fragments ranging from 180–1000 bp (e.g. 300 bp, 600 bp) can be reliably enriched to meet strict size requirements for different sequencing applications.

Easy operation: Identical usage protocol to AMPure XP Beads. No extra workflow optimization required; compatible with all brands of DNA/RNA library construction kits, lowering hands-on experimental barriers. Table 1-2. Reference conditions for DNA fragment size selection

Note: “×” refers to the volume of sample DNA. For example, for 100 μL DNA sample, add 80 μL beads at 0.80× for the first round, then 20 μL beads at 0.20× for the second round. 2.High-efficiency Purification & Outstanding Performance Broad recovery range: The proprietary buffer system enables recovery of DNA fragments as short as 50 bp, with recovery efficiency exceeding 90%, outperforming comparable commercial products. High-purity output: Effectively removes primer dimers, dNTPs, inorganic salts, proteins and other contaminants. The A260/280 ratio of purified DNA remains stable between 1.8–2.0, meeting requirements for high-precision experiments. 3.Cost-effective At equivalent performance, the price is only 60% of imported counterparts. Lead time is shortened to 3–5 working days, supporting cost optimization for large-scale library preparation. 4.Broad Compatibility & Versatile Applications Suitable for restriction digestion, ligation, cloning, NGS library construction and other molecular biology workflows, enabling flexible processing of DNA samples from diverse sources.

Figure 1-77. Fragment size-selection profile of DNA fragments (20–1000 bp, dissolved in nuclease-free water) using bead ratios ranging from 0.8×/0.2× to 0.45×/0.15×. Customer Publications 1.Nature Communications: YTHDF2 enhances anti-tumor immunity of CD8 T cells by coordinating epigenetic and transcriptional networks (DOI: 10.1038/s41467-024-53997-6)

Figure 1-78. UcallM® DNA Clean Beads were used to purify and size-select PCR products during ATAC-seq library construction in this study. 2.Cell Death & Differentiation: Dual regulatory mechanism of Hnrnpk during embryonic limb bud development (DOI: 10.1038/s41418-023-01207-z)

Figure 1-79. UcallM® DNA Clean Beads were applied for size-selection of PCR amplicons in ATAC-seq library prep to capture DNA fragments of 250–350 bp (left). In Hi-C experiments, the beads were used for DNA purification after ligation at a bead ratio of 1.8× (right).