UcallM® FECTMAX PEI Transfection Reagent

PEI is a water-soluble cationic high-molecular-weight polymer with positive charges. It readily binds negatively charged nucleic acids to form complexes, which enter cells via endocytosis. The “proton sponge effect” facilitates endosomal escape and ultimately delivers DNA into the nucleus. PEI transfection reagent is a transient transfection reagent featuring low cytotoxicity, high efficiency, cost-effectiveness and broad applicability. It is widely used for suspension and adherent cell lines including HEK293, U937 and HepG2.

Notes 1.For optimal transfection efficiency, seed cells to reach 50–70% confluency on the day of transfection. 2.Once thawed from −20 °C, PEI solution may be temporarily stored at 4 °C. Avoid repeated freeze-thaw cycles. 3.For most cell types, 3.0 μL PEI reagent per 1 μg DNA yields high transfection efficiency. Ratios between 1:1 and 1:5 can be tested for further optimization.