UcallM® FECTMAX RNP Transfection Reagent

UcallM® FECTMAX RNP Transfection Reagent is an innovative delivery reagent based on cationic lipid nanoparticles, engineered for efficient intracellular delivery of functional proteins into eukaryotic cells. Positively charged nanoparticles form stable non-covalent complexes with target proteins through electrostatic interactions. Attracted by negatively charged cell membranes, complexes are internalized via membrane fusion or clathrin-mediated endocytosis, then released into the cytosol through endosomal escape, preserving protein bioactivity and function. This reagent exhibits low cytotoxicity, high delivery efficiency and broad compatibility.

Applications 1.Intracellular delivery of functional proteins Targeted delivery of signaling modulators (kinases, transcription factors), fluorescently labeled proteins such as GFP fusion proteins. 2.Delivery of genome-editing tools Efficient delivery of CRISPR-Cas9 ribonucleoprotein complexes (RNPs), base editors (BE3) and other large editing machineries to reduce off-target risks. Notes 1.For optimal transfection efficiency, seed cells to reach 70–80% confluency on the day of transfection. 2.Perform complex formation in serum-free, antibiotic-free medium; complete medium containing serum can be used afterwards. Remove growth medium before adding complexes. Serum-containing medium may be added 6 h post-transfection depending on cell type.