U-Blot® LEF-1 Rabbit pAb

U-Blot® LEF-1 Rabbit pAb
U-Blot® LEF-1 Rabbit pAb
$0.00
SKU: WP26780

Specification: 50µL/100µL

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

Product Overview

Catalog No.
WP26780
SKU
WP26780
Category
Polyclonal antibodies
Product Type
Other
Size
50µL/100µL
Stock Status
In stock
Available Stock
999
Minimum Order Quantity
1

Storage & Compliance

Research Use Only
Yes

Additional Specifications

Target
LEF-1
Reactivity
Human, Mouse, Rat
Applications
WB
MW(Calculated)
44kD
MW(Observed)
44kD
Host Species
Rabbit
Isotype
IgG
Conjugate/Modification
Unmodified
Recommended Dilution Ratio
WB 1:5000-1:20000
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
Clonality
Polyclonal
Immunogen
--
Specificity
Endogenous
Gene Name
LEF1
Protein Name
Lymphoid enhancer-binding factor 1
Other Name
LEF1; / Lymphoid enhancer-binding factor 1; / LEF-1; / T cell-specific transcription factor 1-alpha; / TCF1-alpha
Species
Human
Gene ID
51176
Uniprot
Q9UJU2,
Species
Mouse
Gene ID
16842
Uniprot
P27782,
Species
Rat
Gene ID
161452
Uniprot
Q9QXN1
Cellular Localization
Nucleus
Tissue Expression
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
Signaling_pathway
Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Basal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway
Research Areas
>>Wnt signaling pathway; / >>Hippo signaling pathway; / >>Adherens junction; / >>Melanogenesis; / >>Cushing syndrome; / >>Alcoholic liver disease; / >>Salmonella infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Pathways in cancer; / >>Colorectal cancer; / >>Endometrial cancer; / >>Prostate cancer; / >>Thyroid cancer; / >>Basal cell carcinoma; / >>Acute myeloid leukemia; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Arrhythmogenic right ventricular cardiomyopathy
Function
Alternative products:Additional isoforms seem to exist,Domain:Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.,Function:Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subcellular location:Found in nuclear bodies upon PIASG binding.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.,tissue specificity:Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.,

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Keywords:Polyclonal antibodies