U-Blot® GSDMD C-terminal Rabbit pAb

U-Blot® GSDMD C-terminal Rabbit pAb
U-Blot® GSDMD C-terminal Rabbit pAb
$0.00
SKU: WP26782

Specification: 50µL/100µL

Stock: In stock

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U-Blot® GSDMD C-terminal Rabbit pAb

Product Overview

Catalog No.
WP26782
SKU
WP26782
Category
Polyclonal antibodies
Product Type
Other
Size
50µL/100µL
Stock Status
In stock
Available Stock
999
Minimum Order Quantity
1

Storage & Compliance

Research Use Only
Yes

Additional Specifications

Target
GSDMD
Reactivity
Human
Applications
WB
MW(Calculated)
53kD
MW(Observed)
53kD
Host Species
Rabbit
Isotype
IgG
Conjugate/Modification
Unmodified
Recommended Dilution Ratio
WB 1:500-1:2000
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
Clonality
Polyclonal
Immunogen
--
Specificity
The antibody detects endogenous full lenth and c-ternal fragment of gsdmd protein,
Gene Name
GSDMD
Protein Name
Gasdermin domain-containing protein 1,Gasdermin-D,GSDMD,GSDMD-CT,GSDMD-NT,GSDMD_HUMAN
Other Name
--
Species
Human
Gene ID
79792
Uniprot
P57764
Species
--
Gene ID
--
Uniprot
--
Species
--
Gene ID
--
Uniprot
--
Cellular Localization
[Gasdermin-D]: Cytoplasm, cytosol . Inflammasome . In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. .; [Gasdermin-D, N-terminal]: Cell membrane; Multi-pass membrane protein . Secreted . Released in the extracellular milieu following pyroptosis. .; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol .
Tissue Expression
Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.
Signaling_pathway
Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway
Research Areas
>>Neutrophil extracellular trap formation; / >>NOD-like receptor signaling pathway; / >>Salmonella infection
Function
Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms), CASP4, CASP5 or CASP8 relieves autoinhibition and is sufficient to initiate pyroptosis (PubMed:26375003, PubMed:29898893, PubMed:32109412). Cleavage by CASP1 and CASP4 is not strictly dependent on the consensus cleavage site on GSDMD but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412). Cleavage by CASP8 takes place following inactivation of MAP3K7/TAK1 by Yersinia toxin YopJ (By similarity). Cleavage at Asp-87 by CASP3 or CAPS7 inactivates the ability to mediate pyroptosis (PubMed:28392147, PubMed:28045099).

Tech Support

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Keywords:Polyclonal antibodies