UcallM® Zero TOPO-Blunt Simple Cloning Kit

The UcallM® Zero TOPO-Blunt Simple Cloning Kit enables a highly efficient one-step cloning strategy. Unlike conventional cloning workflows, restriction digestion, ligase treatment or end modification are not required. Blunt-end PCR products can be efficiently cloned into vectors within 5 minutes, greatly improving the success rate and convenience of gene cloning. Whether for routine gene cloning, high-throughput screening or expression vector construction, this kit delivers a “hassle-free, no-waiting, high-fidelity” experimental experience.

Experimental Workflow

Product Highlights 1.Ultra-fast cloning with one-step workflow 2.High cloning efficiency and transformation rate 3.Simple operation and broad compatibility Applications 1.High-throughput gene cloning and library construction 2.Rapid assembly of ready-to-use expression vectors

Application Reference Nat Commun: Transcriptome-wide study of alternative polyadenylation reveals new categories of cancer-susceptibility genes (DOI: 10.1038/s41467-024-46064-7) This study systematically analyzed the heritable effects mediated by alternative polyadenylation (APA) across 22 cancer types for the first time.

Figure 1-74. In this research, the TOPO-Blunt zero-blunt cloning kit was applied in 3' RACE (Rapid Amplification of cDNA Ends) experiments. RACE-PCR amplicons were cloned into the PCE2 vector for subsequent Sanger sequencing.