UcallM® Super PiggyBac Transposase

The PiggyBac transposon employs a “cut-and-paste” transposition mechanism. Super PiggyBac transposase recognizes inverted-terminal-repeat (ITR) sequences and mediates efficient genomic integration of cargo sequences at TTAA sites. It accepts large-size DNA cargo and enables footprint-free excision after integration.

Experimental Workflow

(Quoted from GEN)

Key Advantages 1.High transposition efficiency across diverse cell types 2.Tolerates large-size cargo inserts 3.Precise genomic integration plus footprint-free reversible excision Applications 1.Stable-cell-line generation and high-throughput functional-genomics screening (gene over-expression, functional validation) 2.Cell reprogramming and iPSC-genome engineering 3.Vector construction for cell-therapy and gene-therapy research Application Case Nat Biomed Eng: Transient Epigenome Editing Using RNA-LNP for the Treatment of Metabolic Liver Disease in Mice

Figure 1-75. In this study, the PiggyBac transposon system was employed to generate reporter cell lines. It functions for stable genomic integration, reporter system construction and selection marker establishment.